Tupol method

TUPOL METHOD

Developed by Roy (1974). It is made of polythene.

  • Roy (1974) used polythene tubes named “Tupol” for freezing semen at -196⁰C.
  • One end of the tube is immersed in the diluted semen and the other end is attached with the syringe and the semen is sucked into the tube.
  • Then 3-4 inches pieces are cut with electrically heated copper with which seals the cut ends.
  • Insemination is done by cutting one end of the tube and taking the semen in the syringe or glass catheter as per the liquid semen insemination.
  • There is no measure to keep the length of the tube uniform and the sealing is not very satisfactory.
  • More over sealing is done by means of heat, so certain numbers of sperms are damaged at both ends.
  • Therefore volume of semen and number of sperm per dose of insemination is affected.
  • However this method is used in places where other facilities are not available.
Last modified: Monday, 4 June 2012, 10:24 AM