Interpretation of Results
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After incubation observe slides for a definite sharp precipitin line between antigen and hyper immune serum.
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It will be useful to help observe positive reaction between antigen and sera sample.
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The precise "Zone of Equivalence" where there is a perfect concentration of both antibody and antigen in solution, precipitation reactions are occurs and Specific precipitin lines develop within 24-48 hrs.
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This test can be used to determine the relationship betweeen antigens in solution.
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If two antigen wells and one antibody well are to be setup as in fig to show the relationship.
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Three basic patterns of results are observed in agar gel precipitation test-
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Last modified: Friday, 29 October 2010, 5:32 AM