Interpretation of Results

INTERPRETATION

  • After incubation observe slides for a definite sharp precipitin line between antigen and hyper immune serum.
  • It will be useful to help observe positive reaction between antigen and sera sample.
  • The precise "Zone of Equivalence" where there is a perfect concentration of both antibody and antigen in solution, precipitation reactions are occurs and Specific precipitin lines develop within 24-48 hrs.
  • This test can be used to determine the relationship betweeen antigens in solution.
  • If two antigen wells and one antibody well are to be setup as in fig to show the relationship.
  • Three basic patterns of results are observed in agar gel precipitation test-
    • Reaction Identity
      • The precipitation appears as a continuous line in the form of an angle between those two wells.
    • Reaction of Non Identity
      • When two antigens do not contain any common antigenic determinant , the two lines are formed independently and cross without any interaction.
    • Reaction of Partial Identity
      • If some of the determinants are common they give a continous line of identity with spur formation,a partial identity exists, indicating that one antigen possesses epitope not present in other.
    • The coggin's test is a gel diffusion test used to detect antibodies against equine infectious anemia virus in horse serum.
Last modified: Friday, 29 October 2010, 5:32 AM