Veterinary Preventive Medicine-II

DIAGNOSIS

Samples to be collected

• Based on characteristic symptom and lesions
• Nasal, ocular discharges, blood, spleen, lymph nodes, lungs, and liver
  • Virus isolation can be carried out in porcine cell lines using homogenates of spleen and tonsil.
  • The cultures are examined for virus growth by immunofluorescence or immunoperoxidase staining; positive isolates are further characterized by the use of monoclonal antibodies and by partial genetic sequencing.
  • Polymerase chain reaction protocols for the identification of CSFV nucleic acid have now gained international acceptance and are being used in several laboratories, both for detection of the agent and differentiation from ruminant pestiviruses.
  • Antigen-capture enzyme-linked immunosorbent assays ( ELISAs ) are also useful for herd screening, but should not be used on a single animal basis.
  • Serological tests - VNT, ELISA