FPT 121: 1.Using an acid and its salt or a Base and its salt

1.Using an acid and its salt or a Base and its salt

1.Using an acid and its salt or a Base and its salt

Prepare a buffer composed of an acid and its salt by dissolving the acid form of the buffer in about ~60% of the water required for the final solution volume.

Adjust the pH using a strong base, such as NaOH. When preparing a buffer composed of a base and its salt, start with the base form and adjust the pH with strong acid, such as HCl.

After the pH is correct, dilute to just under the final solution volume.

Check the pH and correct if necessary, then add water to the final volume.

Last modified: Friday, 16 December 2011, 6:07 AM

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      - Definition
      - Preparation of Buffer
      - 1.Using an acid and its salt or a Base and its salt
      - 2. Using the buffer pKa
      - 3. Two solution method
      - 4. Use of available Table
      - i.Acetate buffer solution pH 3 - 6
      - ii.Phosphate buffer pH=7.00
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Principles of Biochemistry (2+1)

1.Using an acid and its salt or a Base and its salt

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