Principles of Biochemistry (2+1)

Prepare the following solutions

• (1) 0.1M acetic acid
• (2) 0.1M sodium acetate (tri-hydrate) (13.6 g / l)
• Mix in the following proportions to get the required pH.
• Check the pH and correct if necessary.

  pH	Vol. of 0.1M acetic acid	Vol. of 0.1M sodium acetate
  3	98.23 ml	1.77 ml
  4	84.70 ml	15.30 ml
  5	35.70 ml	64..0 ml
  6	0.2ml	94.8ml