FPT 121: Definition

Definition

Buffers are composed of weak acids and their salts, or weak bases and their salts.

If the protonated form is uncharged, it is an acid (like acetic acid), and its unprotonated form is a salt (e.g., sodium acetate).

Conversely, if the unprotonated form is uncharged it is a base (like Tris base), and its protonated form is a salt (e.g., TrisHCl).

Last modified: Monday, 14 November 2011, 6:09 AM

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      - Definition
      - Preparation of Buffer
      - 1.Using an acid and its salt or a Base and its salt
      - 2. Using the buffer pKa
      - 3. Two solution method
      - 4. Use of available Table
      - i.Acetate buffer solution pH 3 - 6
      - ii.Phosphate buffer pH=7.00
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Principles of Biochemistry (2+1)

Definition

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