ii.Phosphate buffer pH=7.00

ii. Phosphate buffer pH=7.00

Prepare the following solutions

  • 1. 0.1M NaOH
  • 2. 0.1 M potassium dihydrogen phosphate
  • Add 29.1 ml of 0.1 molar NaOH to 50 ml 0.1 molar potassium dihydrogen phosphate.
  • Check and adjust the pH and make up the volume to 100ml with distilled water

Alternate method

  • Dissolve 0.120g of sodium dihydrogen phosphate and 0.089g of disodium hydrogen phosphate in 60ml of water.
  • Check and adjust the pH and make up the volume to 100ml with distilled water.

Last modified: Monday, 19 March 2012, 6:05 AM