Reagents

Reagents

  • 30% KOH
  • 95% H2SO4 : Add 100ml of Conc. H2SO4 to 5ml of D/W in a beaker and cool the resultant solution.
  • 0.2% anthrone solution: Dissolve 0.2 g of anthrone in 100 ml of 95% H2SO4. (The reagent is not stable in solution for extended periods of time and is best made up daily as needed).
  • Stock standard glucose: Dissolve 100mg glucose in 100ml of distilled water in a volumetric flask. 1ml of this solution contains 1 mg of glucose.
  • Working standard glucose: Dilute 5ml of the above solution to 50 ml in distilled water in a volumetric flask. ml of this solution contains 100 µg of glucose.
Last modified: Monday, 14 November 2011, 5:57 AM