Principles of Biochemistry (2+1)

Calculation

Calculate the amount of glycogen present in the sample using the following equation.

 

 mg. of glycogen in 100g of the  sample  =     U x 0.05 x 50 x100x100  

                                                     ----------------------------------                                                                            S x 0.5x 10x1.11 x 1

   U    =  The absorption of unknown test solution

   S    =  The absorption of working standard glucose. 

  1.11 = The factor determined by morris for the conversion of

             glucose to glycogen