Determination of glucose and glycogen

Determination of glucose and glycogen

  • Add into a test tube 0.5ml of the above sample solution.
  • Into a second tube add 0.5 ml of the working glucose standard.
  • Make up the volume to 1ml with distilled water.
  • Into a third tube add 1ml of D/W, this constitutes the blank.
  • While submerged in cold water add 4 ml of the anthrone reagent into each tube and mix well.
  • Immediately cool the tubes in a bath containing cold water and read the colour developed in a colorimeter at 630nm.
Last modified: Wednesday, 22 February 2012, 10:08 AM