Principles of Biochemistry (2+1)

                                                           X  x  100  x  100

Amount of protein %   =         ----------------------

                                                                     0.1 x  5 x 1000

X   =  Amount of protein obtained from graph (in mg)

v    = volume of sample used (0.1ml)

V   =  Total volume of supernatant of sample(100ml)

W  =  weight of sample (5g)