Principle
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A chromatographic separation is generally a technique, in which mobile phase, which is passing over a stationary phase, transport different substances with different velocities on the direction of flow.
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Thin layer chromatography is a method of separating a mixture into its different components using a thin layer.
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The stationary phase is a thin layer of adsorbents such as silica gel, silicic acid or cellulose placed on a glass support.
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Separation is made in a chromatographic chamber containing a relevant solvent system (mobile phase).
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As the solvent rises through the adsorbent medium by capillary action, it tends to resolve the components of the sample.
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Electrostatic forces of stationary phase act to retard the component in the sample as the mobile phase rises.
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This and the fact that the component has different solubility in the mobile phase cause the individual components to move at different rates along with the solvent and thus separated.
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Last modified: Monday, 14 November 2011, 5:21 AM