Principle

Principle

  • A chromatographic separation is generally a technique, in which mobile phase, which is passing over a stationary phase, transport different substances with different velocities on the direction of flow.
  • Thin layer chromatography is a method of separating a mixture into its different components using a thin layer.
  • The stationary phase is a thin layer of adsorbents such as silica gel, silicic acid or cellulose placed on a glass support.
  • Separation is made in a chromatographic chamber containing a relevant solvent system (mobile phase).
  • As the solvent rises through the adsorbent medium by capillary action, it tends to resolve the components of the sample.
  • Electrostatic forces of stationary phase act to retard the component in the sample as the mobile phase rises.
  • This and the fact that the component has different solubility in the mobile phase cause the individual components to move at different rates along with the solvent and thus separated.
Last modified: Monday, 14 November 2011, 5:21 AM