• Petroleum ether (boiling point 60-80ºC): diethyl ether:
• Glacial acetic acid = 80:20:10 – freshly prepared

2. Standards

•  Hydrocarbon
  • n-hexadecane
  • n-octadecane
• Cholesterol esters
  • Cholesterol oleate
  • Cholesterol stearate
• Vit. A ester
  • Vit. Acetate
  • Vit. A palmitate
• Triglycerides
  •  Glycerol trioleate
  •  Glycerol tristearate
  •  Glycerol tripalmitate
•  Sterol a. Cholesterol
•  Free fatty acids
  • Oleic acid
  • Palmitic acid
  • Stearic acid

3. Sample

• Cod liver oil, Shark liver oil, fish body oil, fat extract from fish and other seafood.

4. Detection agents

• 2.7 dichlorofluoresene – 2g/1 in 95% v/v ethanol

• Iodine