VGO 511: Veterinary Andrology and Reproductive Techniques (1+1)

MILLOVANOV’S RESISTANCE TEST

• This test assesses the ability of spermatozoa to withstand the action of 1 per cent Sodium chloride solution.
• The resistance is denoted as the millilitre of 1per cent Sodium chloride solution required to stop the progressive motility of all spermatozoa in 0.02 ml of semen.

Procedure

• Pipette out 0.02 ml of semen into a 200 ml capacity flask
• Add 1% sodium chloride solution volumes each of 10 ml from a pipette
• Assess the progressive motility of semen after each addition 10 ml of sodium chloride.
• Sodium chloride is added at intervals till the progressive motility of spermatozoa is ceased
• Good quality semen shows ‘R’ value to be not less than 5000.

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