VGO 511: Veterinary Andrology and Reproductive Techniques (1+1)

PHYSICAL METHODS OF EVALUATION OF SEMEN

Objective method of measurement of motility

• Computer assisted semen analysis system ( phase contrast microscope, cameras and control monitor with personal computer with 640 kb CPU co-processor and digitizer with 256 grey valves terminal printer) will yield exact data regarding motility, concentration, velocity of cells, ratio of live cells, quantity of required diluent and straws.
• It has the capacity of storing entire production information.

Impedance change frequency

• This technique involves the passage of electric current with very low voltage in the freshly collected semen.
• This impedance caused by motile spermatozoa is recorded in the meter.
• Semen charged with electric current during impedance change frequency test is safe and can be used subsequently for Artificial Insemination.

Filtration through Sephadex

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