Cold shock resistance test

COLD SHOCK RESISTANCE TEST

Introduction

  • This test is done to assess the fertilizability/preservability (5°C) of the semen of a particular bull.
  • The spermatozoa are subjected to unfavourable condition such as cold (0°C).
  • The percentage of resistant live sperm is counted after cold shock.
  • There is a good correlation between percentage of sperms which withstand cold shock and motility after 5 days of preservation under chilled condition (5°C).

Materials required

  • Test tube
  • Semen sample
  • Microscope
  • Crushed ice
  • Eosin and Nigrosin stain

Procedure

  • Examine the live sperm percentage of the fresh semen immediately after collection.
  • Take one ml of freshly collected in a test tube and place it in a beaker containing crushed ice (0⁰C) for 10 minutes.
  • Determine the percentage of live sperm and motility after cold shock.
  • Compare the percentage of live sperm in fresh semen sample and semen after expose to crushed ice.
  • Then estimate the storage ability and freezability of spermatozoa.
Last modified: Monday, 11 June 2012, 11:32 AM