VGO 511: Veterinary Andrology and Reproductive Techniques (1+1)

Different tests

DIFFERENT TESTS TO ASSESS THE SPERM QUALITY

• There are different test are available to assess the semen quality and classified under following headings.

I. Macroscopic examination

• Volume
• Colour
• Consistency/density
• Presence of foreign materials
• Gross motility

II Microscopic examination

• Mass activity
• Individual motility
• Concentration estimation
• Live and dead sperm estimation
• Abnormal sperm estimation
• Acrosome integrity evaluation

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III. Biochemical tests

• pH estimation
• Methylene blue reduction test (MBRT)
• Hypoosmotic swelling test (HOST)
• Fructolytic index
• Oxygen uptake/ utilization test
• Pyruvate utilisation test
• Glutamic oxaloacetic transaminase (GOT) activity
• Hyaluronidase activity
• Resazurin test
• Buffering capacity test
• Alkaline & acid phosphatase test
• Millovanov’s resistance test

IV.Resistance to environment

• Low temperature storage / resistance to cold shock
• High temperature viability test
• Resistance to sodium chloride / millovanov’s resistance test (R-test)

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V. Physical evaluation

• Objective measurement of motility
• Impedance change frequency
• Filteration through sephadex

VI.Chemical Evaluation

• DNA estimation
• Ascorbic acid content
• Potassium & sodium estimation
• Fructose level in semen
• Citric acid

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VII.Post- thaw evaluation of semen

• Post – thaw motility
• Post – thaw acrosomal integrity
• Post – thaw acrosomal enzyme release
• Post – thaw incubation survival or viability at 37 ° c

VIII. Biological Test

• Zona free hamster egg penetration test (HEPT)
• Zona binding assay (ZBA)

Even though various tests are available only few important tests are commonly followed as routine tests. Other tests are followed only during specific conditions.

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