VGO 511: Veterinary Andrology and Reproductive Techniques (1+1)

PRELIMINARY STORAGE AND POST FREEZE EXAMINATION

Preliminary storage

• After primary freezing the straws are plunged into the plastic goblet filled with liquid nitrogen.
• When the boil–off ceases the goblets are transferred to canister of frozen semen container for storage at – 196 ° C.

Post-freezing examination

• Post thaw motility of samples is assessed after 24 hours of storage.
• Samples having less than 50 percent progressive motility (under phase contrast microscope) are discarded.
• Good sample are transferred to permanent storage containers for preservation.

• Post thaw motility evaluation - done after 24 hours of preliminary storage.
• Minimum post thaw motility to accept a semen sample - 50%.