Ampoule method
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Method of freezing
- This method was developed in USA by Macpherson (1954), Vandemark and Kinney (1954).
- Immediately after equilibration the semen of o.5-1.0 ml is placed in previously marked glass ampoules
- These ampoules were sealed over flame leaving 0.5 ml air space
- The ampoules are placed in ethyl alcohol or acetone bath at 5⁰C
- Small quantity of solid carbondioxide the temperature was brought from 5⁰C to -15⁰C (at the rate of 1-2⁰C/min).
- Now more solid carbondioxide is added to bring the temperature to -79⁰C (at the rate of 4-5⁰C/min).
- Six to eight ampoules are arranged in clips or metal cane and each cane is marked with identification of individual bull.
- Then the ampoules are stored in solid carbondioxide at -79⁰C or in liquid nitrogen at-196⁰C.
Thawing
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The ampoule is thawed in iced water for 5-7 minutes.
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- The seal was broken and the semen is aspirated in glass catheter for insemination.
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Advantages
- Hence the semen is stored in the sealed ampoule the chance of contamination is less
- Identification is easy as the details are printed over the ampoule and the cane.
Disadvantages
- As the semen is frozen in larger volume, the freezabilty and fertility is less.
- The ampoules occupies more space in the cane
- About 8-10% of the semen is lost during handling, thawing and inseminating.
- The insemination is done with glass catheters which is having disadvantages.
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Last modified: Monday, 4 June 2012, 11:22 AM
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