Evaluation of semen

EVALUATION OF SEMEN

  • Immediately after collection the collection vial is taken out of AV and the rims are covered with aluminium foil.
  • The details of the collection are pasted on the vial (bull No., ejaculate number etc).
  • It is placed in a 34⁰C water bath in the pass box (The lab is tightly sealed at the time of processing to maintain hygiene. So the pass box helps to transport of semen from collection yard to lab. But the pass box should not be opened at both the sides simultaneously).
  • Inside the lab the semen is evaluated for volume, color, consistency and presence of foreign matter and then placed in a water bath of 34 ⁰ C.
  • This temperature has to be maintained till the final dilution of semen.
  • One drop of semen is placed on glass slide and mass activity recorded.
  • Samples showing more than +3 are taken for estimating concentration of spermatozoa.
  • The concentration is estimated by using colorimeter or photometer.
  • The semen is diluted with diluent in 1:1 ratio.
  • The initial motility is assessed by placing a drop of 1:1 diluted semen on a warm slide and placing a cover slip on it.
  • The initial motility is estimated by using phase contrast microscope.
  • A sample with 70% and above initial motility is selected for further processing.
  • The entire process of above mentioned semen evaluation has to be completed within 5-7 minutes.

The semen in collection vial has to be kept at 34⁰C water bath from collection to 1:1 dilution. After 1:1 dilution it is kept outside the water bath to bring the temperature to 22⁰C before final dilution.

The minimum requirement for fresh semen to be processed are as follows

  • Volume - 2.5 ml
  • Concentration - 500 million and above
  • Initial motility - 70%

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Semen evaluation

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Last modified: Monday, 4 June 2012, 11:21 AM