VLD 421: Veterinary Clinical Biochemistry and Laboratory Diagnosis-II (0+1)

PROCEDURE

• The test should be carried out between 20⁰C and 25⁰C. (At higher temperature false positive reactions caused by Auto - Agglutination may be observed).
• Place a drop of suspected serum on a glass slide/plate.
• Place a drop of colored antigen next to the serum sample.
• The drops are then mixed gently with stirring-rod and spread to a circular area of approximately 1.5cm diameter.
• After a few minutes, in case of positive reaction ,clumping takes place (Agglutination).
• Confirm the reaction with suitable positive and negative controls.

Analysis

+++	A strong positive reaction visible within 3 seconds
++	Appearance of medium sized particles within 2-3    minutes
+	Appearance of very tiny powdery paticles    developing in 4- 5 minutes
Negative	Absence of agglutination.