Biochemical Techniques and Instrumentation

3.2.1. Enzyme Linked Immuno Sorbent Assay (ELISA)

Enzyme-linked immunosorbent assay (ELISA) is a biochemical technique mainly used in immunology to detect the presence of an antibody or an antigen in a sample. It is used for the detection and quantification of peptides, proteins, antibodies, enzymes and hormones. Peter Perlmann and Eva Engvall of Sweden invented this technique in 1971.