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2.2.5.2. Electroporation
2.2.5.2. Electroporation
Electroporation is another method of gene transfer . It utilizes a series of short electrical pulses to make the membrane porous and permeable to DNA incorporation.
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Embryos and sperms can be electroporated.
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It is less labour intensive and does not require special expertise for gene transfer as needed in the case of microinjection .
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It is easier to do this in spermatozoa than in embryos, which possess tough chorion.
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The gene transfer efficiency and integration rate do not differ much between electroporation and microinjection methods.
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In zebra fish 0.1 milli second pulses of 125/cm for batches of 200 eggs.
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This technique has been tested on medaka, zebrafish, common carp, catfish and loach.
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When compared with different methods of gene transfer (microinjection, sperm-mediated, chromosome mediated and through electroporation), despite lower survival, electroporation technique ensures a higher transfer efficiency.
Last modified: Tuesday, 26 June 2012, 11:56 AM