Biotechnology and Bioinformatics (1+1)

2.2.6.3. Western blotting

It is used for identifying and characterizing specific gene products.

• It involves the transfer of protein s from acrylamide gels to nitrocellulose membrane by electrophoresis.
• The membrane is then probed with an antibody to detect the protein of interest.

In situ hybridization is the hybridization of nucleic acids within cytological preparations. This method shows the localization of transgenes.