Biotechnology and Bioinformatics (1+1)

2.2.6.4. PCR amplification

It is based on repeated cycles of denaturation, annealing of oligonucleotide primer s complementary to the gene, and primer extension by Taq polymerase . The amplified fragment can then be recognized as a discrete fragment on a gel or on a southern blot.

Expression assays are aimed at detecting the presence of reporter gene products such as CAT and ß- gal in host cells.