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2.1.3.4 Yeast cloning vectors
· Yeast cloning vectors are the carrier DNA molecules into yeasts.
· Yeasts are eukaryotes.
· Yeast artificial chromosome (YACS) are most sophisticated yeast vectors.
· They have centromeric and telomeric region of a chromosome. These regions are needed to allow chromosome to be replicated in yeast cells.
· Due to origin of replication that is present, replication of DNA occurs. These elements are placed in single DNA fragment which can be used as vector to clone foreign DNA into yeasts.
· The advantages of YAC include very large piece of DNA can be cloned. Only single copy of YAC is present per cell.
Last modified: Tuesday, 19 June 2012, 10:48 AM