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2.1.4.2. Cloning in Eukaryotes
2.1.4.2. Cloning in Eukaryotes
E. coli offers limitation to its use in cloning eukaryotic genes as splicing of mRNA cannot take place. Therefore, eukaryotic cells are used to clone and express eukaryotic genes. Among eukaryotes, DNA cloning has been done mostly in yeast cells.Yeast is becoming an important organism for the commercial production of medically important proteins such as viral vaccines. It is of great value as an organism for basic research, for producing heterologous proteins and as a vehicle for cloning large segments of DNA.
Last modified: Friday, 22 June 2012, 5:19 AM