Biotechnology and Bioinformatics (1+1)

2.2.6.1. Southern blot hybridization

It is the most widely used method.

• In this method, fragments of DNA generated by restriction digestion, are subjected to agarose gel electrophoresis .
• The separated fragments are then transferred to a nitrocellulose or nylon membrane by a blotting technique.
• The DNA of interest can be detected by hybridizing the membrane to a radioactive probe , which bears the same homology as the DNA.