3.6.1.3 Dehydrogenation of isocitrate to oxalosuccinate

3.6.1.3 Step 3: Dehydrogenation of Isocitrate to Oxalosuccinate and then to α-ketoglutarate

Isocitrate undergoes dehydrogenation in the presence of isocitrate dehydrogenase to form oxalosuccinate.The linked oxidation of isocitrate proceeds almost completely through the NAD+ dependent enzyme isocitrate dehydrogenase. There follows decarboxylation of oxalosuccinate to α-ketoglutarate, also catalyzed by isocitrate dehydrogenase. A CO2 molecule is liberated. Mn2+(or Mg2+) is an important component of the decarboxylation .


Isocitrate dehydrogenase

Isocitrate + NAD+ Oxalosuccinate + NADH+ H+

Oxalosuccinate α-ketoglutarate + CO2
Last modified: Thursday, 10 November 2011, 9:57 AM