Principles of Genetics and Breeding

9.1.5 Non cryogenic preservation (short-term)

• This is the short duration preservation for 3-4 days. By reducing the metabolic activity life is increased. The milt sample is diluted with extender and stored in a thermocol chamber with ice or in refrigerator at 4ºC.
• Care should be taken to avoid freezing the samples. In general, sperm samples should be stored in shallow containers with the lids loosely attached to allow oxygenation of the sperm cells during storage.
• This is helpful for designing different sib breeding out of different male and female traits. Extender C is found more easy and effective for such preservation.
• Milt samples are brought to room temperature before inseminating the egg sample for better result.