Principles of Genetics and Breeding

9.1.7.5 Storing

• Liquid nitrogen (-196ºC) is the most commonly used cryogen.
• The freezed milt straws/visitubes are immediately immersed in liquid nitrogen and kept undisturbed.
• The evaporation loss of liquid nitrogen is compensated by regular filling to the container.
• To manage cryobanks efficiently, it is essential to keep comprehensive records of all stocks preserved.