Principles of Genetics and Breeding

9.1.10 Liquid nitrogen safety precautions

• Always wear insulated gloves and safety glasses when handling LN₂.
• Never place objects cooled by LN₂ against unprotected skin.
• Use only containers designed for use with LN₂.
• Use proper transfer equipment to move and handle samples. Never use hollow rods or tubes as dipsticks, because LN₂ can be driven out of the open end.
• Never cover or plug the opening of any LN₂ container. Considerable pressure will build up as the LN₂ vaporizes.
• Always allow proper venting. In addition, nitrogen gas can collect in closed areas, displacing the air, and creating a potential suffocation hazard. Work in well-ventilated areas.