Principles of Biochemistry (2+1)

Installation of the gel

i. After the stacking gel has polymerised, remove the comb without disturbing the shapes of the well carefully install the gel after removing the clips, agar etc. in the electrophoresis apparatus.

ii. Fill it with electrode buffer and remove any trapped air bubbles at the bottom of the gel.

iii. Connect the electrode at the top (the stacking gel end of the gel) and turn on the DC power briefly to check the electrical circuit.The electrode buffer and the plates can be kept cool using a suitable facility so that heat generated during the run is dissipated and does not affect the gel and resolution.