Principles of Biochemistry (2+1)

Staining and Destaining

Staining

i. After the run was completed, carefully removed the gel from between the plates and immerse in staining solution for at least 3 hours overnight with uniform shaking.

ii. The proteins absorb the Coomasie billiant blue.

Destaining

i. Transfer the gel to a suitable container with at least 200-300 ml destaining solution and shake gently and continuously.

ii. Dye that is not bound to proteins is thus removed.

iii. Change the destaining solution frequently, particularly during initial periods, until the background of the gel is colorless.

iv. The proteins fractionated into the band are colored blue.

v. When the proteins of minute quantities are stained faintly, stop the destining process to visualize as many bands as possible.