VMC 221: Veterinary Immunology and Serology (1+1)

ANTIGEN-ANTIBODY BINDING TESTS

Diagnostic serological techniques fall into three broad categories.

• Primary binding tests directly measure the binding of Ag to Ab.
• Secondary binding tests measure the results of Ag-Ab interactions in vitro. These are less sensitive but easier to perform.
• Tertiary binding tests measure the actual protective action of antibodies in an animal. Most complex of the tests.