VMC 221: Veterinary Immunology and Serology (1+1)

Introduction- Primary binding tests

INTRODUCTION

• Primary binding tests are performed by allowing Ag and Ab to combine and then measuring the amount of immune complexes formed.
• Radioisotopes, fluorescent dyes or enzymes are used as labels to identify one of the reactants.
  • Radio immuno assay (RIA)
  • Immunofluorescence assays
  • Enzyme immune assays (EIAs)
  • Immunoperoxidase test (IPT)
  • Western blotting (Immunoblotting)