Indirect ELISA

INDIRECT ELISA

  • Antibody can be detected using indirect ELISA.
  • Serum or some other test sample containing primary antibody is added to an antigen coated microtitre plate well.
  • After an incubation period free primary antibody is removed by washing, the presence of antibody bound to the antigen is detected by adding an enzyme tagged secondary antibody or antispecies antibody or antiglobulin which binds to the primary antibody.
  • After an incubation period any free secondary antibody is washed away and a substrate for the enzyme is added.
  • The amount of colored reaction product formed is measured by specialized ELISA readers .

Indirect-ELISA
Last modified: Thursday, 26 August 2010, 7:15 AM