VMC 221: Veterinary Immunology and Serology (1+1)

DIRECT ELISA (To detect Antigen)

• Antigen is attached to the solid phase such as 96 well polystyrene plates. After washing enzyme labeled antibody is added.
• After incubation period and it is washed and substrate system is added.
• Development of colour is read using ELISA reader (figure).
• Reading is taken at a particular wave length depending on the substrate used.