VMC 221: Veterinary Immunology and Serology (1+1)

SANDWICH TEST

• It is a double layer procedure designed to visualize specific antibody.
• First, fix the cell with ethanol to prevent antibody being washed away during the test, and then treat with a solution of antigen.
• After washing, a fluorescein labeled antibody to the particular antigen would then be added to locate those cells which had specifically bound to the antigen.
• The name of the test derives from the fact that antigen is sandwiched between the antibodies present on the cell substrate and added as the second layer.