VMC 221: Veterinary Immunology and Serology (1+1)

AVIDIN BIOTIN ELISA

• Enzymes may inhibit antibody activity or lose enzymatic activity in the process of conjugating them to antiglobulin.
• One alternative is to use biotin and its specific binding protein avidin.
• Biotin can bind to protein (antibody) without affecting their biological activity.
• Avidin binds very strongly and specifically to biotin and may be conjugated with enzymes.