VGO 511: Veterinary Andrology and Reproductive Techniques (1+1)

GLYCEROL

Glycerol is the most widely and commonly used cryoprotective agent for bull spermatozoa.

• It was the spectacular discovery of Polge in 1949 that showed that the death of spermatozoa could be avoided if the cells were suspended in a medium containing glycerol.
• The possible modes of action of glycerol are:
  • Modifies the size and shape of ice crystals formed.
  • Binds water and decrease freezing point of solution and less ice is formed.
  • Acts through salt buffering mechanism.
  • Reduces solute concentration.
  • Prevents denaturation of proteins and rupture of plasma membrane.