VGO 511: Veterinary Andrology and Reproductive Techniques (1+1)

Precautions in the preparation of semen diluents

PRECAUTIONS IN DILUENT PREPARATION

• Only double glass distilled water, fresh eggs and pure chemicals are used for preparing diluents.
• Care is taken not to rupture the yolk membrane before it becomes free of white.
• The yolk membrane should be retained with filter paper and discarded.
• Enough buffer solution is prepared in bulk, sterilised and kept in refrigerator.
• Diluents are prepared on the previous day itself of collection and kept in a refrigerator until used before dilution, the temperature of the diluent should be 30 ° C and this is achieved by keeping it in a water bath for 15-20 minutes before dilution.
• The semen is always kept at 30 ° C during evaluation.
• Dilution should be done within 10 minutes after collection.
• Glycerol when added should be warmed to 40 ° C. After addition, the glycerol should be mixed well with the diluents by stirring gently with a sterile glass rod for 20 minutes.
• Always use sterilised glassware during the entire operation.