Diluents for liquid semen

DILUENTS FOR LIQUID SEMEN

Sodium citrate egg yolk diluent

Composition

  • Tri-Sodium citrate dihydrate : 9.375g
  • Glycine : 12.5g
  • Glucose anhydrous : 18.75g
  • Distilled water : 1 litre
  • Penicillin G sodium : 1 lakh I.U. / vial
  • Dihydrostreptomycin : 1g / vial
  • Add 20 ml of egg yolk with 80 ml of buffer to prepare the diluent.

Tris buffer

Composition

  • Tris (hydroxy methyl aminomethane) – 0.3M: 30.4812g
  • Citric acid monohydrate : 17.0g
  • Fructose : 12.5g
  • Distilled water : up to 850 ml
  • Penicillin G sodium or ] : 10 lakhs / vial
  • Benzyl Penicillin ]
  • Dihydrostreptomycin : 1g / vial
  • The pH should be adjusted to 6.75

Reconstituted skim milk diluent

  • For dilution of semen either whole milk or skim milk or reconstituted skim milk can be used. These were heated to 92 ° C to 95 ° C for 8 to 10 minutes in a water bath.
  • Too much heating is harmful because of its effect on the lactose. Ultra-heat-treated long-life milk could also be used wherein sterilisation and heating is not necessary.
  • A small volume of egg yolk is added to milk, which improves the sperm survival, and inactivates the spermicide in milk.

Composition
Last modified: Monday, 4 June 2012, 10:15 AM