Preparation of diluent

PREPARATION OF DILUENT

  • The egg yolk is prepared as per the standard procedure

(Click here for video demonstrationView video)

  • For 1 litre of diluent, 200 ml of egg yolk is required
  • The egg yolk is added and mixed by using magnetic stirrer
  • The antibiotics are also added while stirring with egg yolk
  • Care should be taken while stirring that air bubbles should not be formed by over speed of the stirrer
  • The pH of the diluents should be 6.7-6.8.

Egg yolk is added @ 20%.

Last modified: Monday, 11 June 2012, 11:36 AM