VGO 511: Veterinary Andrology and Reproductive Techniques (1+1)

DILUENTS FOR FROZEN SEMEN

Sodium citrate egg yolk diluent

Composition

• Diluent for frozen semen : 420 ml
• Glycerol : 80 ml
• Glycerol is warmed to about 40 ° C before addition and mix gently for 20 minutes.

Tris buffer

Composition

• Tris (hydroxy methyl amino methane)- 0.3M : 30.4812g
• Citric acid monohydrate : 17.0g
• Fructose : 12.5g
• Glass distilled water : 850 ml
• Benzyl Penicillin : 1 lakh I.U. / vial
• Dihydrostreptomycin : 1g / vial
• From the basic diluent take : 336 ml
• Glycerol : 64 ml
• Egg yolk : 100 ml
• This should be mixed gently for 20 minutes.

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Reconstituted skim milk diluent

Composition

• No difference in fertility was found between semen from the same source extended with heated skim milk to which 5 – 10 per cent glycerol can be added.
• It is better maintained for several days at 5 ° C with milk glycerol extender.
• The semen can be added directly to glycerolized milk yolk extender.

Note: Even though so much diluents are present for buffalo semen, the sodium citrate egg yolk diluent is a better one.

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