The animal should be restrained in a service crate.
The perineal region of the animal should be washed with running tap water to remove the dung and dirt. The water should be wiped off.
The inseminators hand is covered with disposable rubber sleeve.
The lubrication is done with non-irritant soap and the hand is passed into the rectum.
The excessive dung inside the rectum is removed. But complete removal is not advisable because a minimum level of dung is essential for better operation.
Frequent removal of hand from the rectum while removing dung also should be avoided because it will lead to ballooning of the rectum.
Vulva and vulval lips are carefully wiped or if necessary, washed and then wiped dry with cotton or a paper towel.
The cervix is grasped with gloved hand and the external os is located.
If the external os is hard to locate, the cervix may be hold by the fingers and the thumb is placed over the external os.
The vulval lips are widened apart by an attendant and the insemination gun is inserted through the vulva and vagina and into the external os of the cervix
If folds of vaginal wall interfere, the cervix is pulled or pushed forwarded to straighten the lumen of the vagina.
By manipulation of the cervix with the hand in the rectum, the insemination catheter is passed just through the annular rings of the cervix.
The semen is deposited through the cervix into the body of the uterus and the remainder in the internal os of the cervix, while the catheter is withdrawn.
Semen is expelled slowly to avoid sperm losses in the catheter.
A common error is to penetrate the catheter beyond the body of the uterus into the uterine horns.
Advantages of recto-vaginal technique of insemination
Deposition of semen at proper site is ensured without loss of semen
Chance of infection are minimized and fertility rate is high compared to speculum method.
Injury/bleeding caused while using speculum is avoided in this method
Estrum is confirmed while examining the animal per rectum
Inseminating pregnant animal is also avoided because the rectal palpation of genital organs rules out pregnancy.
Participants