Biochemical Techniques and Instrumentation (1+2)

2.1.2. Types of chromatographic system

2.1.2. Types of chromatographic systems

The selection of chromatographic system is made so that the compounds have different distribution coefficients. This is achieved by

1. Adsorption chromatography : An adsorption equilibrium between a stationery solid and a mobile liquid phase
2. Partition chromatography: A partition equilibrium between a stationery liquid or semi-liquid and a mobile liquid phase
3. Gas-liquid chromatography: A partition equilibrium between a stationery liquid and a mobile gaseous phase
4. Ion-exchange chromatography: An ion exchange equilibrium between an ion exchange resin stationery phase and a mobile electrolyte phase
5. Exclusion chromatography: An equilibrium between a liquid phase inside and outside a porous structure or molecular seive
6. Affinity chromatography: An equilibrium between a macromolecule and a small molecule for which it has a high biological specificity