Site pages
Current course
Participants
General
Topic 1
Topic 2
Topic 3
Topic 4
Topic 5
Topic 6
Topic 7
Topic 8
Topic 9
Topic 10
Topic 11
Topic 12
Topic 13
Topic 14
Topic 15
Topic 16
Topic 17
Topic 18
Topic 19
Topic 20
Topic 21
Topic 22
Topic 23
Topic 24
Topic 25
Topic 26
2.3.5. Eluent buffers
There are anionic and cationic buffers for the elution of compounds.
The pH of the buffer selected as eluent should be one pH unit above or below the isotonic point of the molecule. The initial pH and ionic strength should allow the binding of the molecules to the exchanger. A buffer of the lowest ionic strength should be initially used as it ensures that minimum binding of contaminants to the exchanger. If gradient elution is used, initial conditions should allow all the sample compounds to bind at the top of the column. |
Last modified: Wednesday, 7 December 2011, 12:06 PM