Site pages
Current course
Participants
General
Topic 1
Topic 2
Topic 3
Topic 4
Topic 5
Topic 6
Topic 7
Topic 8
Topic 9
Topic 10
Topic 11
Topic 12
Topic 13
Topic 14
Topic 15
Topic 16
Topic 17
Topic 18
Topic 19
Topic 20
Topic 21
Topic 22
Topic 23
Topic 24
Topic 25
Topic 26
2.2.3.4. Detectors
Components collected on each tube are measured by direct spectrophotometric techniques. In sophisticated equipments, the outlet of the column is connected to an on-line detector, which monitors the components passed through a flow cell. These monitors give a continuous readout in the form of chromatogram that helps to collect the fractions of interest. The most popular detector is an absorbance spectrophotometer, which detects components by their ability to absorb light at defined wavelengths.
|
Last modified: Thursday, 10 November 2011, 11:00 AM