VGO 511: Veterinary Andrology and Reproductive Techniques (1+1)

MILOVANOV'S METHOD/CARBONATE-PHOSPHATE METHOD

This diluent was developed by Milovanov (USSR) during 1959.

• It is also called as “carbonate phosphate method”.
• Composition of this media is as follows

Part A

S.No.	Components	Quantity
1	Potassium dihydrogen phosphate	72 mg
2	Distilled water	10 ml

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Part B

S.No.	Components	Quantity
1	Sodium citrate	2.025 gm
2	Glucose	0.570 gm
3	Sodium bicarbonate	0.125 gm
4	Penicillin G sodium	60,000 mg
5	Dihydro streptomycin sulphate	0.100 gm
6	Sulphanilamide	0.300 gm
7	Distilled water	90,000 ml

• Mix Part A and Part B in the ratio of 1:9.

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Part C

• Add 11 ml of egg yolk to the above solution (AB).
• The antibiotics are added as per the schedule.
• One ml of the diluted semen are sealed off in glass ampoules and stored at 20-25^oC.

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